Cryoprobe for 600 MHz Spectrometer at Griffith University
Medium field (500/600 mHz) spectrometers play a critical role in
biotechnology research by providing systems to examine small biomolecules,
less than about 15 kDaltons in molecular size. The medium field systems
have substantially lower sensitivity than 900 MHz systems but recent
advances in NMR probe technology, in particular the development of
cryoprobes, has allowed marked sensitivity gains to be obtained when
such probes are retrofitted to existing spectrometers. The upgrade
to the 600 MHz system at Griffith provides up to four times improvement
in the sensitivity of the spectrometer.
A cryoprobe can increase sensitivity by reducing the system electronic
noise, thereby increasing the signal-to-noise ratio. By freezing the
probe to liquid Helium temperatures a significant amount of 'noise'
is removed. An increase in sensitivity by a factor of 2-4 can reduce
the time taken to accumulate a data set by a factor of 4-16. The addition
of a cryoprobe is therefore a cost-efficient mechanism for increasing
the usage capacity of the spectrometer at Griffith University and
significantly addresses the problem of limited sample amounts because
of the vast increase in sensitivity that this device provides.